Thermophilic bacteria isolated from hot springs of Himachal Pradesh were screened for xylanase activity and production. Of the 38 strains screened, 19 were xylanase positive and among them 5 promising strains with significant hydrolytic capacity were considered for further investigation. Under submerged conditions, xylanase production of these isolates ranged from 0.64 to 2.36 IU.ml-1 at 72 h using birch wood xylan as substrate. These isolates were further confirmed for the presence of xylanase gene (700bp) and protein profile showing high density activity bands between 75 to 90 KDa. The maximum level of xylanase production was noted with Bacillus licheniformis KBFB3, which also exhibited relatively more activity in a broad pH range between 4.5 and 8.5 and temperature up to 80°C. From the above results, it is vivid that the highly thermostable and alkali tolerant xylanase of B.licheniformis KBFB3 could find its application in efficient biomass valorization.
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Madras Agricultural Journal (MAJ)
Madras Agricultural Students Union
Tamil Nadu Agricultural University
Coimbatore - 641
003
Tamil Nadu, India.
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